NANOVIBRONIX ULTRASOUND TECHNOLOGY ENHANCES HUMAN SKIN REGENERATION


This research explored the potential of NanoVibronix technology to enhance epidermal and dermal structures in human skin explants. The research was performed at BIO-EC laboratory, Paris, FRANCE

Background

The goal – explore the potential of NanoVibronix technology in stimulating regeneration of epidermal and dermal structures of human skin explants.
The evaluation methods – Observation of general morphology and visualization of different biological precursors or indicators after immuno- staining.

Methods

Explants were put into six wells culture plate and treated by one of four protocols:

  • No treatment – control
  • Stimulation in continuous mode – Continues mode – stimulation for10 minutes per day. Treatment was repeated at days: D1, D2, D3, D6,D7, D8 and D9.
  • Stimulation with intermittent period – Intermittent mode – total of 6minutes of situation including 3 cycles with stimulation for 2 minutes and intermission of 2 minutes. Treatment was repeated at days: D1, D2, D3, D6, D7, D8 and D9.
  • Stimulation by topical reference product (Retinol) – Retinoic Acid – applied topically to explants with 2 mg per explants. Products have been applied on D0, D3, D6 and D8.

Results

  • Immunostaining

Treatment with intermittent mode induces marked increase cytokeratin 14, slight increase in neutral GAGs and Collagen III, while a decrease in Ki67 and Collagen IV was observed

  • Quantification of epidermal thickness and CK14 intensity

Treatment with intermittent mode induces a significant increase of approx. 75% and 80% in epidermal thickness and CK14 expression in comparison to the untreated explants, respectively.

Conclusions:

According to these results, Nanovibronix stimulation with intermittent mode induces a very clear epidermal stimulation with a good cellular morphology compared to the Retinol Tx.
Stimulation with intermittent mode induces:

  • A clear basal cytokeratin 14 over expression.
  • A slight neutral GAGs over expression.
  • A slight collagen III over expression.
  • A clear decrease of cells in mitosis.
  • No collagen IV over expression.
  • No blood vessels stimulation.

These results show that treatment with Nanovibronix’s ultrasound technology in intermittent mode has a clear epidermal activity and a slight neutral GAGs stimulation close to the dermal epidermal-junction indicating enhancement of skin tissue regeneration.


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