The goal – explore the potential of NanoVibronix Surface Acoustic Waves (SAW) Ultrasound technology in stimulating cell migration, which is a crucial step during wound healing process.
Human Keratinocytes (NIK) were maintained in Epilife growth medium with human growth factor supplement (HGFS). The cells were grown to 90-95 % confluent in 35 mm cell culture dishes, which were subsequently used for the scratch assay. The effect of the SAW on cell migration was studied by comparing the migration rate of the treated cells vs. the non-treated cells.
In the treatment group, cells were treated 5 minutes every 24 hours using the NanoVibronix device. Prior to the treatment, the cells were washed twice with PBS, and then maintained in 1.5 ml Epilife growth medium. In each experiment, triplicate sample and control were included. The setup of the experiment was shown in Figure 1.
A total of 3 trials were conducted. For each trial, a triplicate for both treated and non-treated group was performed. In general, the cells were treated continuously for four days from day 0 to day 3. Every 24 hours, the migrated cells were imaged and the gap areas of the scratch were measured.
For data analysis, total of 9 data samples were collected for each day, and these data were combined and used for statistic analysis.
The cell migration rate of treated and untreated samples was compared by measuring the relative gap area, which is defined as the ratio of the gap area at Tt (At) to the gap area at To (A0) (Relative gap area=At/A0 ×100%). In order to meet the statistic requirement, data collected in three trials were combined and analyzed using Prism Statistic software. The statistic results are plotted as Relative Gap Area vs. Time (Figure 2) and summarized in Table 1.